d-Idose, d-Iduronic Acid, and d-Idonic Acid from d-Glucose via Seven-Carbon Sugars

Sucrose/D-Glucose/ D-Fructose

1. Bottle 1 with approx. 0.5 g lyophilizate, consisting of: citrate buffer, pH approx. 4.6; β-fructosidase, approx. 720 U 2. Bottle 2 with approx. 7.2 g powder mixture, consisting of: triethanolamine buffer, pH approx. 7.6; NADP, approx. 110 mg; ATP, approx. 260 mg; magnesium sulfate 3. Bottle 3 with approx. 1.1 ml suspension, consisting of: hexokinase, approx. 320 U; glucose-6-phosphate dehydr...

Synthesis of a 3-deoxy-D-manno-octulosonic acid (KDO) building block from D-glucose via fermentation.

Herein we report the first synthesis of a 3-deoxy-D-manno-octulosonic acid (KDO) building block starting from glucose through pathway engineering of Escherichia coli and subsequent chemical modifications to provide an alternative method to produce KDO, found in plant and bacterial oligosaccharides.

D-Isocitric acid

Mix**, wait for the end of the reaction (approx. 10 min) and read the absorbances of the solutions (A2). If the reaction has not stopped after 10 min, continue to read the absorbances at 2 min intervals until the absorbance increases constantly over 2 min. Determine the absorbance differences (A2-A1) for both, blank and sample. Subtract the absorbance difference of the blank from the absorbance...

Melting behaviour of D-sucrose, D-glucose and D-fructose.

The melting behaviour of d-sucrose, d-glucose and d-fructose was studied. The melting peaks were determined with DSC and the start of decomposition was studied with TG at different rates of heating. In addition, melting points were determined with a melting point apparatus. The samples were identified as d-sucrose, alpha-d-glucopyranose and beta-d-fructopyranose by powder diffraction measuremen...

THE OXIDATION OF HEXOSAMINES : d-GLUCOSAMINE AND d-GLUCOSAMINIC ACID